The enzyme production for bioethanol described by this dataset is intended for use in the conversion of biomass into bioethanol fuel, which is suitable for combustion in automobile engines. This process is applicable in the production of renewable and sustainable energy resources intended to replace or supplement traditional fossil fuels. When using this data set, consider the eight technological configurations determined by the pretreatment technology for bioethanol production. The following technological steps are consistent across the literature, but variations in actual flows and parameter values must be acknowledged. Global datasets have been utilized; therefore, adapt the data accordingly if applying to a specific region. The 'Combustion of bioethanol in car' process has been incorporated from the GREET database, and must be considered for life cycle inventory (LCI) comparisons. When interpreting the robustness of modeling results, apply Monte Carlo simulations and other analyses such as discernibility, global sensitivity, and scenario variation to manage uncertainties.