The glyphosate produced through the glycine method is primarily used as a broad-spectrum herbicide for agricultural purposes. It may also be utilized in non-agricultural areas such as public and private gardens, aquatic systems, and industrial sites. This chemical is effective in controlling weeds and is therefore often applied in the cultivation of crops that are genetically modified to be glyphosate resistant. When using this data set for life cycle assessment, it is important to account for potential allocation procedures especially if the production system includes multi-output processes. Users should ensure that emissions and resource consumption are assigned appropriately to the production of glyphosate, according to the specific methodological guidelines of their LCA study. Depending on the context of the study, the use phase of glyphosate and its related environmental impacts, including potential water pollution, should be modelled with attention to geographical and agronomic conditions.